E4906
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 | SDS-PAGE (4-20%) of enterokinase (Cat. No. E4906) under reducing conditions and stained with Coomassie blue. |
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E4906
Sigma
Enterokinase from bovine intestine
BioUltra, recombinant, expressed in Escherichia coli, ≥20 units/mg protein, ≥95% (SDS-PAGE)
| Synonym: | Enteropeptidase |
| CAS Number: | 9014-74-8 |
| Enzyme Commission (EC) Number: | 3.4.21.9 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00131020 |
Description
| Application | Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags. |
| Typical conditions for fusion protein cleavage: Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween™ 20 Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix Incubate reaction mixture at ~25 °C for 16 hours | |
| Unit Definition | One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C. |
| Physical form | Supplied as a solution in 20mM Tris-HCl; 200mM NaCl, 2mM CaCl2, and 50% glycerol |
| Physical properties | 28 kDa light chain form |
Properties
| recombinant | expressed in Escherichia coli |
| grade | BioUltra |
| assay | ≥95% (SDS-PAGE) |
| packaging | vial of ~0.2 unit |
| shipped in | wet ice |
| storage temp. | −20°C |
